Reporter

Part:BBa_K1713020:Design

Designed by: Zhu qi   Group: iGEM15_HZAU-China   (2015-09-14)

dBroccoli

This part is a RNA aptamer that can be used for the genetic enconding of fluorescent RNA. It can binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1Himidazol-5(4H)-one) (DFHBI-1T),and shows green fluorescence in cells.(ex = 465nm,em = 535nm)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part was synthesized in TSINGKE and standardized by ourselves.

References

Filonov G S, Moon J D, Nina S, et al. Broccoli: Rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution.[J]. Journal of the American Chemical Society, 2014, 136.